Epub 2018 Aug 2. Email: RCole@jhmi.edu. Yoder JB, Ben-Johny M, Farinelli F, Srinivasan L, Shoemaker SR, Tomaselli GF, Gabelli SB, Amzel LM. of Biophysics and Biophysical Chemistry. The instruments in the Department of Chemistry mass spectrometry facility are available to all who require them in the course of their work, both from within the Johns Hopkins community, and from outside sources (including academia, commercial, government institutions, high schools, etc.). Ca2+-dependent regulation of sodium channels NaV1.4 and NaV1.5 is controlled by the post-IQ motif. Translational Proteomics Laboratory General Information | Self-Checker | Donate and Lend Support | Staff Appreciation | Get Email Alerts. The Chemistry Department Mass Spectrometry Facility Structural analyses of NudT16-ADP-ribose complexes direct rational design of mutants with improved processing of poly(ADP-ribosyl)ated proteins. Advanced Clinical Chemistry Diagnostics Laboratory. Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase. Contact. To establish an account, please download and complete the PDF Document: Chemistry Core Facility Access Form and return it to the facility manager. The Sidney Kimmel Comprehensive Cancer Center, and the Conte Digestive Diseases Basic and Translational Research Core Center. Please continue to call your providers with health concerns. The JHU Department of Chemistry Mass Spectrometry Facility (MSF) is a component of the department’s core instrumentation service. Molecules. © 2020 Johns Hopkins University, Zanvyl Krieger School of Arts & SciencesThe Chemistry Department Mass Spectrometry FacilityRemsen Hall B133400 N. Charles St., Baltimore, MD 21218Tel: 410-516-5552 | Fax: 410-516-8420mass.spec@jhu.edu, Sample Preparation and Submission Guidelines. The Johns Hopkins Germ-free Core is ~1500 square feet with 3 animal rooms, dedicated autoclave and two work rooms. For more information or to enquire about the use of our instruments, please contact Dr. Phil Mortimer at the Mass Spectrometry Facility, 410-516-5552, mass.spec@jhu.edu. The Johns Hopkins AIMS Core provides comprehensive service in MALDI tissue imaging to investigators at Johns Hopkins and outside. Phone: 410-614-6968. Tel: 410-516-5552 We provide specialty … We look forward to working with you in the future. Following successful completion of a training session and receipt of the Facility Access Form, users will be granted access credentials and usage privileges. The most up-to-date information on instrument availability can be found on our Instrument Status and News page. Maheshwari S, Shimokawa C, Rudzka K, Kline CD, Eipper BA, Mains RE, Gabelli SB*, Blackburn N, Amzel LM. Email: RCole@jhmi.edu. Users are required to treat all instruments with care and respect and to maintain their competence in order to retain usage privileges. Tel: 410-516-5552. mass.spec@jhu.edu. The Johns Hopkins Applied Imaging Mass Spectrometry (AIMS) Core/Service Center provides rapid matrix-assisted laser desorption/ionization (MALDI) imaging at high spatial resolution, which includes sample preparation, on-tissue digests and derivatizations, and data analysis. doi: 10.3390/molecules24030496. Our clinical mass spectrometry laboratory is equipped with a variety of chromatographic equipment and mass analyzers, including multiple high-resolution accurate mass instruments. 3400 N. Charles St. Baltimore, MD 21218. We continue to monitor COVID-19 cases in our area and providers will notify you if there are scheduling changes. Home; About Us; Faculty; Reference Chemistry; Clinical Mass Spectometry; Instrumentation Miller MS, Maheshwari S, Shi W, Gao Y, Chu N, Soares AS, Cole PA, Amzel LM, Fuchs MR, Jakoncic J, Gabelli SB. Our clinical mass spectrometry laboratory is equipped with a variety of chromatographic equipment and mass analyzers, including multiple high-resolution accurate mass instruments. Where a user is considered to be causing a detrimental effect on a particular instrument, additional training sessions may be required by the Facility Manager. Johns Hopkins University School of Medicine. Fax: 410-516-8420. mass.spec@jhu.edu. We are located on East Baltimore campus of Johns Hopkins University School of Medicine. The facility’s primary role is to support chemical research conducted within the department, but it is also available for use by researchers from other JHU departments and divisions, along with those from external institutions and commercial organizations. 2019 Jan 30;24(3). 2019 Jan 30;24(3). We primarily work with blood or urine specimens, but we have the capability to work with alternative samples such as tissue, DBS, or sputum. Kristine Glunde, M.S., Ph.D.​DirectorEmail: kglunde1@jhmi.eduTelephone: 410-614-2705 (office), 410-614-7959 (AIMS Core), Dr. Kristine GlundeThe Russell H. Morgan Department of Radiology and Radiological ScienceThe Johns Hopkins University School of MedicineOffice: Traylor Building, Room 203, Rutland Avenue, Baltimore, Maryland 21205AIMS Core: Cancer Research Building II, Room LB03D/E, Baltimore, Maryland 21231, to Radiology and Radiological Science Main Menu, The Johns Hopkins Applied Imaging Mass Spectrometry – AIMS Core/Service Center, The Center for Image-Guided Animal Therapy, Bruker Rapiflex MALDI TOF/TOF instrument for high-throughput MALDI imaging, HTX M5 sprayer for accurate robotic spraying of enzymes and matrices, Leica cryostat for MALDI imaging-compatible cryosectioning, Slide scanner for histology and immunohistochemistry co-registered with MALDI imaging, High-end workstation with SCiLS lab for data analysis, MALDI imaging compatible cryosectioning in gelatin and other MALDI compatible cryomedia, MALDI imaging sample preparation of formalin-fixed, paraffin-embedded tissue sections, On-tissue digestions including for tryptic peptides and glycans, On-tissue derivatizations for metabolite imaging, Robotic matrix application with HTX M5 sprayer, High-throughput multiplexed MALDI imaging of up to 5,000 biomolecules at once, High spatial resolution MALDI imaging of 20 micron pixel size or better, MALDI imaging covering maximum area of regular microscopy slide (75 millimeters by 25 millimeters), Customized development of MALDI imaging protocols, Targeted MALDI imaging of drugs, drug metabolites, imaging agents, contrast agents or other agents, Discovery MALDI imaging of metabolites, lipids, peptides, intact proteins, tryptic peptides and glycans, On tissue MS/MS for analyte identification in imaging or profiling mode, Data analysis: segmentation analysis, pathology-guided analysis and statistical analysis with dedicated software package (SCiLS Lab). All users (including those who wish to submit samples for analysis on “full-service” instruments) will need to establish an account with the department for instrument access and billing purposes. Johns Hopkins Fundamentals – A closer look at basic biomedical research. The instruments in the Department of Chemistry mass spectrometry facility are available to all who require them in the course of their work, both from within the Johns Hopkins community, and from outside sources (including academia, commercial, government institutions, high schools, etc.). It is used to identify, quantify and characterize proteins and their post-translational modifications, expressed in well-characterized protein fractions from cancerous cells, tissues or body fluids. Kim Phan joins the lab. Proteomics, Johns Hopkins. We are providing in-person care and telemedicine appointments. We welcome enquiries into the use of these instruments. The facility is normally open Monday–Friday, 9 a.m.–6 p.m. Director Robert Cole Ph.D. Instrument training is free and is normally conducted on Wednesdays. B13, Remsen Hall, Homewood Campus Cell. Column chromatography and gel electrophoresis-based one and two-dimensional separations of protein complexes coupled to mass spectrometry are used. Sci Rep. 2019 Apr 11;9(1):5940. doi: 10.1038/s41598-019-39491-w. Miller MS, Maheshwari S, Shi W, Gao Y, Chu N, Soares AS, Cole PA, Amzel LM, Fuchs MR, Jakoncic J, Gabelli SB. The Johns Hopkins Mass Spectrometry and Proteomic Facility. Chu N, Salguero AL, Liu AZ, Chen Z, Dempsey DR, Ficarro SB, Alexander WM, Marto JA, Li Y, Amzel LM, Gabelli SB*, Cole PA. Akt Kinase Activation Mechanisms Revealed Using Protein Semisynthesis. The Chemistry Department Mass Spectrometry Facility. 2019 Apr 3;10(1):1514. doi: 10.1038/s41467-019-09570-7.

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